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nonspecific antibody binding  (Boster Bio)


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    Boster Bio nonspecific antibody binding
    Nonspecific Antibody Binding, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nonspecific+antibody+binding/Anti-RAB45+Rabbit+Monoclonal+Antibody/pmc13021461-181-2-8
    Average 94 stars, based on 2 article reviews
    nonspecific antibody binding - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Binding Assay:

    Article Title: Short-term toxicity of dibutyl phthalate to mice intestinal tissue.
    Article Snippet: .. The sections were placed in 0.01 M citric acid buffer (pH 6.0) and then heated in a microwave oven at 800 W for 3 min followed by 400 W for 10 min. Nonspecific antibody binding was blocked by incubating with normal bovine serum albumin (BSA; diluted and ready to use, AR1006, Wuhan Boster Biological Technology, China) for 20 min at RT. ..

    Article Title: CPEB1 drives ferroptosis–neuroinflammation crosstalk in temporal lobe epilepsy via the SIRT1–NRF2 acetylation axis
    Article Snippet: The sections were then incubated in 0.3% Triton X-100 solution (Beyotime, China) at room temperature for 15 minutes to enhance membrane permeability. .. To block nonspecific antibody binding, 10% goat serum (Boster, China) was applied at 37 °C for 30 minutes. ..

    Article Title: Expression of cyclooxygenase-2 and vascular endothelial growth factor-C correlates with lymphangiogenesis and lymphatic invasion in human gastric cancer.
    Article Snippet: Background.. Recent observations have suggested that overexpression of cyclooxygenase-2 (COX-2) promotes tumor lymphangiogenesis through an upregulation of vascular endothelial growth factor-C (VEGF-C) expression.. It is unclear whether this mechanism also acts in gastric cancer.

    Blocking Assay:

    Article Title: CPEB1 drives ferroptosis–neuroinflammation crosstalk in temporal lobe epilepsy via the SIRT1–NRF2 acetylation axis
    Article Snippet: The sections were then incubated in 0.3% Triton X-100 solution (Beyotime, China) at room temperature for 15 minutes to enhance membrane permeability. .. To block nonspecific antibody binding, 10% goat serum (Boster, China) was applied at 37 °C for 30 minutes. ..

    Saline:

    Article Title: Expression of cyclooxygenase-2 and vascular endothelial growth factor-C correlates with lymphangiogenesis and lymphatic invasion in human gastric cancer.
    Article Snippet: Background.. Recent observations have suggested that overexpression of cyclooxygenase-2 (COX-2) promotes tumor lymphangiogenesis through an upregulation of vascular endothelial growth factor-C (VEGF-C) expression.. It is unclear whether this mechanism also acts in gastric cancer.

    Incubation:

    Article Title: Expression of cyclooxygenase-2 and vascular endothelial growth factor-C correlates with lymphangiogenesis and lymphatic invasion in human gastric cancer.
    Article Snippet: Background.. Recent observations have suggested that overexpression of cyclooxygenase-2 (COX-2) promotes tumor lymphangiogenesis through an upregulation of vascular endothelial growth factor-C (VEGF-C) expression.. It is unclear whether this mechanism also acts in gastric cancer.



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    Image Search Results


    Figure 9. CD8+ T cell depletion ameliorates psoriasis and arthritis in humanized mice. (A) PsA mice were injected with anti-CD8 or isotype control anti- bodies at the time of PBMC injection. At day 30 after transfer, mouse PBMCs were stained with a DEAD/LIVE marker and nonspecific binding blocked with human TruStain FcX Fc receptor blocking solution. The percentage of CD19–CD3+CD8+ T cells was defined with FlowJo to determine the efficiency of CD8+ T cell depletion. (B and C) hu-PsA mice injected with CD8 antibodies did not develop psoriasiform lesions or ankle swelling, compared with mice injected with isotype control antibodies. (D) Epidermal hypertrophy and numerous dermal infiltrating cells were observed in the skin of hu-PsA mice injected with isotype control antibodies. (E) Infiltrating CD3+ T cells (red) and Ki67+ proliferating cells in skin of humanized mice. (F) CD8+ T cell depletion significantly reduced epidermal thickness in hu-PsA mice. (G) Pannus areas were visualized in hu-PsA treated with isotype control or CD8+ T cell–depleting antibodies. The dashed line outlines pannus areas. (H) Proliferative type 1 T cells (CD3, red; T-bet, green; Ki67, white) and proliferating T cells (CD3, red; Ki67, white) were decreased in CD8+ T cell–depleted mice. Representative images of Alcian Blue and H&E stain were taken with an Olympus VS120 scanner. Scale bars: 1,000 μm. IF 3 × 3 mosaic images were taken (original magnification, ×200) with a Zeiss Axioplan microscope and recorded with a Hamamatsu camera. (I) Quantitation of CD3+ T cells, CD3+Ki67+ proliferating T cells, and CD3+T-bet+ type 1 T cells in ankle synovial tissue of hu-PsA mice. Scale bar: 200 μm. n = 5 mice reconstituted with PBMCs and sera from different patients with PsA in each group. Significance was calculated with 2-way ANOVA and Tukey’s multiple comparison test. *P ≤ 0.05; **P ≤ 0.005; ****P ≤ 0.0001.

    Journal: JCI insight

    Article Title: Psoriatic arthritis subtypes are phenocopied in humanized mice.

    doi: 10.1172/jci.insight.178213

    Figure Lengend Snippet: Figure 9. CD8+ T cell depletion ameliorates psoriasis and arthritis in humanized mice. (A) PsA mice were injected with anti-CD8 or isotype control anti- bodies at the time of PBMC injection. At day 30 after transfer, mouse PBMCs were stained with a DEAD/LIVE marker and nonspecific binding blocked with human TruStain FcX Fc receptor blocking solution. The percentage of CD19–CD3+CD8+ T cells was defined with FlowJo to determine the efficiency of CD8+ T cell depletion. (B and C) hu-PsA mice injected with CD8 antibodies did not develop psoriasiform lesions or ankle swelling, compared with mice injected with isotype control antibodies. (D) Epidermal hypertrophy and numerous dermal infiltrating cells were observed in the skin of hu-PsA mice injected with isotype control antibodies. (E) Infiltrating CD3+ T cells (red) and Ki67+ proliferating cells in skin of humanized mice. (F) CD8+ T cell depletion significantly reduced epidermal thickness in hu-PsA mice. (G) Pannus areas were visualized in hu-PsA treated with isotype control or CD8+ T cell–depleting antibodies. The dashed line outlines pannus areas. (H) Proliferative type 1 T cells (CD3, red; T-bet, green; Ki67, white) and proliferating T cells (CD3, red; Ki67, white) were decreased in CD8+ T cell–depleted mice. Representative images of Alcian Blue and H&E stain were taken with an Olympus VS120 scanner. Scale bars: 1,000 μm. IF 3 × 3 mosaic images were taken (original magnification, ×200) with a Zeiss Axioplan microscope and recorded with a Hamamatsu camera. (I) Quantitation of CD3+ T cells, CD3+Ki67+ proliferating T cells, and CD3+T-bet+ type 1 T cells in ankle synovial tissue of hu-PsA mice. Scale bar: 200 μm. n = 5 mice reconstituted with PBMCs and sera from different patients with PsA in each group. Significance was calculated with 2-way ANOVA and Tukey’s multiple comparison test. *P ≤ 0.05; **P ≤ 0.005; ****P ≤ 0.0001.

    Article Snippet: Nonspecific binding was prevented by incubation with 10 μg/mL Fc block (clone 2.4G2, BE0307, RRID:AB_2736987, BioXcell) for 10 minutes on ice.

    Techniques: Injection, Control, Staining, Marker, Binding Assay, Blocking Assay, Microscopy, Quantitation Assay, Comparison